scrapie prions bound to a stainless steel surface. Mol. Med. 5(4):240243. 12. Infectivity of Scrapie Prions Bound to a Stainless Steel Surface. May 1999 · Molecular Medicine. A direct assessment of human prion adhered to steel wire Zobeley E., Flechsig E., Cozzio A., Enari M. & Weissmann C. Infectivity of scrapie prions bound to a stainless steel surface. Mol Med 5, 240243 (1999). [PMC free article] Flechsig E. et al. Transmission of scrapie by steel-surface-bound prions. Mol Med 7, 679684 (2001). [PMC free article]
Nov 01, 2013 · Further investigation of the potential of homogenous photocatalytic oxidation to inactivate prions adsorbed on metal substrates was conducted employing the well accepted prion infectivity assay, based on the intracranial implantation of steel wires. 29 Groups of stainless steel or titanium wires (n:30) were contaminated with the 263K prion strain and were then, treated with 56 g mL 1 Inactivation of Prions and Amyloid Seeds with Hypochlorous Sep 29, 2016 · Bioassay of inactivation of steel-bound scrapie infectivity. To determine the effects of HOCl on steel-bound scrapie infectivity, we implanted single BrioHOCl-treated or mock-treated stainless steel wires into the brains of tg7 mice. Concurrently, we also tested bleach-, NaOH- and Environ LpH -treated wires for scrapie infectivity. Infectivity of Prion Protein Bound to Stainless Steel Stainless steel wire (0.15 x 5 mm) exposed to scrapie-infected mouse brain homogenate and washed extensively with PBS retained the equivalent of about 10(5) LD50 units per segment.
Infectivity of scrapie prions bound to a stainless steel surface. Mol Med 1999 Apr;5(4):240-3 Zobeley E, Flechsig E, Cozzio A, Enari M, Weissmann C The transmissible agent of Creutzfeldt-Jakob disease (CJD) is not readily destroyed by conventional sterilization and transmissions by surgical instruments have been reported. Transmission of Scrapie by Steel-surface-bound Prions The ability of stainless steel surfaces to bind scrapie infectivity has been previously demonstrated by incubating steel wires (5 × 0.15 mm) for 16 h with 10% w/v brain homogenate of terminally scrapie-sick mice, referred to below as standard conditions ().To model the exposure of surgical instruments to infected tissue more realistically, we inserted wire segments for 5, 30 or 120 min Infectivity of scrapie prions bound to a stainless steel Infectivity was estimated from the time elapsing to terminal disease. RESULTS:Stainless steel wire (0.15 x 5 mm) exposed to scrapie-infected mouse brain homogenate and washed extensively with PBS retained the equivalent of about 10(5) LD50 units per segment. Treatment with 10% formaldehyde for 1 hr reduced this value by only about 30-fold.